| Autor: |
M. Njayou, R. Drillien, André Kirn |
| Rok vydání: |
1982 |
| Předmět: |
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| Zdroj: |
Annales de l'Institut Pasteur / Virologie. 133:393-402 |
| ISSN: |
0769-2617 |
| DOI: |
10.1016/s0769-2617(82)80067-1 |
| Popis: |
Summary Knowing that infection of Chinese hamster ovary (CHO) cells with vaccinia virus results within the first two hours in an overall inhibition of protein synthesis, and that failure of vaccinia to multiply in CHO cells could be related to this effect, we set out to show that inhibition of protein synthesis was characterized by a breakdown of polyribosomes and an accumulation of 80 S monosomes which remained associated at high salt concentrations. Moreover, extracts from infected cells were impaired in their capacity to carry out in vitro protein synthesis. The reduced level of in vitro protein synthesis appeared to be mainly due to an inactive ribosomal fraction and to a certain extent to a decreased activity of the ribosomal salt wash fraction. RNA extracted from infected cells was translated efficiently in reticulocyte lysates. Taken together, the results suggest that in vivo polypeptide synthesis is arrested either as a result of the formation of an inactive 80 S initiation complex or because of a defect in a subsequent elongation event. These general features of inhibition of protein synthesis in CHO cells are discussed in relation to those previously reported for vaccinia permissive cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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