Scattering angle resolved optical coherence tomography forin vivomurine retinal imaging
| Autor: | Vikram Baruah, Henry Grady Rylander, Austin McElroy, Nitesh Katta, Michael R. Gardner, Thomas E. Milner |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Nervous system
Materials science genetic structures Aperture media_common.quotation_subject 01 natural sciences Retinal ganglion 010309 optics 03 medical and health sciences 0302 clinical medicine Optics Optical coherence tomography In vivo 0103 physical sciences medicine Contrast (vision) skin and connective tissue diseases media_common Retina medicine.diagnostic_test business.industry eye diseases medicine.anatomical_structure sense organs business Retinal scan 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXI. |
| ISSN: | 0277-786X |
| Popis: | Optical coherence tomography (OCT) retinal imaging contributes to understanding central nervous system (CNS) diseases because the eye is an anatomical “window to the brain” with direct optical access to nonmylenated retinal ganglion cells. However, many CNS diseases are associated with neuronal changes beyond the resolution of standard OCT retinal imaging systems. Though studies have shown the utility of scattering angle resolved (SAR) OCT for particle sizing and detecting disease states ex vivo, a compact SAR-OCT system for in vivo rodent retinal imaging has not previously been reported. We report a fiber-based SAR-OCT system (swept source at 1310 nm ± 65 nm, 100 kHz scan rate) for mouse retinal imaging with a partial glass window (center aperture) for angular discrimination of backscattered light. This design incorporates a dual-axis MEMS mirror conjugate to the ocular pupil plane and a high collection efficiency objective. A muring retina is imaged during euthanasia, and the proposed SAR-index is examined versus time. Results show a positive correlation between the SAR-index and the sub-cellular hypoxic response of neurons to isoflurane overdose during euthanasia. The proposed SAR-OCT design and image process technique offer a contrast mechanism able to detect sub-resolution neuronal changes for murine retinal imaging. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|