| Autor: | Jose‐Vicente Sinisterra, R.M. de la Casa, J.M. Sánchez-Montero |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
chemistry.chemical_classification
biology Triacylglycerol lipase Bioengineering General Medicine equipment and supplies Applied Microbiology and Biotechnology Candida rugosa chemistry.chemical_compound Enzyme Dextran Hydrophily chemistry Biochemistry Biocatalysis biology.protein Organic chemistry Lipase Lactose Biotechnology |
| Zdroj: | Biotechnology Letters. 21:123-128 |
| ISSN: | 0141-5492 |
| Popis: | Replacing the lactose used in the commercial preparation of lipase from Candida rugosa by dextrans with different molecular weight, several preparations with enhanced activities in esterification of (R,S)-ibuprofen in organic medium were obtained. The presence of carbohydrates modifies the microenvironment of the enzyme and maintains the hydration of the biocatalyst. We can modulate the hydrophilic/hydrophobic balance on the surface of the biocatalyst creating non covalent enzyme-dextran complexes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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