In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

Autor:	David Sánchez-Porras, Óscar Darío García-García, Enrique España-Guerrero, Cristina Blanco-Elices, Miguel Angel Martin-Piedra, Ingrid Garzón, Miguel Alaminos, Ricardo Fernández-Valadés, María del Carmen Sánchez-Quevedo, Miguel Mateu-Sanz
Rok vydání:	2020
Předmět:	0303 health sciences Scaffold Chemistry Biomaterial 02 engineering and technology 021001 nanoscience & nanotechnology Regenerative medicine Extracellular matrix 03 medical and health sciences medicine.anatomical_structure Tissue engineering medicine Surface modification General Materials Science Oral mucosa 0210 nano-technology 030304 developmental biology Biofabrication Biomedical engineering
Zdroj:	Materials. 13:1692
ISSN:	1996-1944
DOI:	10.3390/ma13071692
Popis:	Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0f7b6e91cb7dc553e000ed012839c5c3 https://doi.org/10.3390/ma13071692 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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