Application of pharmacia automated FPLC System and PepRPC HR 5/5 bonded phase column for determination of dansylated polyamines

Autor:	L. Holczinger, T. Kremmer, M. Boldizsár
Rok vydání:	1985
Předmět:	Chromatography Organic Chemistry Clinical Biochemistry Spermine Fast protein liquid chromatography Biochemistry Toluene Analytical Chemistry Spermidine chemistry.chemical_compound chemistry Phase (matter) Putrescine Methanol Linear correlation
Zdroj:	Chromatographia. 20:79-82
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02280601
Popis:	The Pharmacia Automatized FPLC System equipped with a PepRPC HR 5/5 bonded-phase column was tested with dansyl polyamines, toluene, dimethyl- and dibutylphthalate, using either isocratic conditions or linear gradient-elution program of methanol in water as the mobile phase. A simple and reproducible method is described for the quantiation of dansylated natural polyamines, such as putrescine, spermidine and spermine originating from P388/S leukemia cells. Comparing the data from analyses performed in parallel by the Automated FPLC/PepRPC HR 5/5, and a Hewlett-Packard Model 1084B/LiChrosorb RP-8 systems a linear correlation has been found, with a regression coefficient of r=0.974.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0f68a8f962816acccd13d37241f5e5f1 https://doi.org/10.1007/bf02280601 Zobrazit plný text záznamu Full text from SpringerLink