Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent

Autor: Vasudeo Zambare, Pradnya P. Kanekar, S. S. Nilegaonkar
Rok vydání: 2007
Předmět:
Zdroj: World Journal of Microbiology and Biotechnology. 23:1569-1574
ISSN: 1573-0972
0959-3993
DOI: 10.1007/s11274-007-9402-y
Popis: The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic observation of longitudinal section of buffalo hide revealed that the epidermis was completely removed and hair was uprooted leaving empty follicles in the hide. The ASP enzyme was stable for one month at ambient temperature between 25–35 °C. Enzymatic dehairing may be a promising shift towards an environment-friendly leather processing method.
Databáze: OpenAIRE