PHOTODYNAMIC TREATMENT OF YEAST WITH CHLOROALUMINUM-PHTHALOCYANINE: ROLE OF THE MONOMERIC FORM OF THE DYE

Autor:	H. J. G. M. De Bont, J. F. Nagelkerke, A. E. Van Gompel, J. Van Steveninck, P. J. van den Broek, Michel Paardekooper
Rok vydání:	1994
Předmět:	chemistry.chemical_compound Monomer chemistry Kluyveromyces marxianus biology Phthalocyanine General Medicine Physical and Theoretical Chemistry biology.organism_classification Photochemistry Biochemistry Yeast Chloroaluminum phthalocyanine
Zdroj:	Photochemistry and Photobiology. 59:161-166
ISSN:	1751-1097 0031-8655
Popis:	The effect of photodynamic treatment on the yeast Kluyveromyces marxianus with aluminum-phthalocyanines has been studied. It was found that the nonsulfonated sensitizer caused light-dependent loss of colony-forming capacity, whereas the mono- and tetrasulfonated forms did not induce loss of clonogenicity. The effect of the nonsulfonated sensitizer increased with longer preincubation periods of cells with the dye. Formation of cellattached, mostly intracellularly localized monomelic sensitizer also increased with time. The amount of cell-bound multimeric nonsulfonated phthalocyanine did not vary with time. Experiments designed to specifically increase the amount of cell-attached monomers led also to an increased photoinactivation of the cells. It is therefore concluded that the photodynamic effect of the nonsulfonated Al-phthalocyanine is mediated by the monomeric form of the dye.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0f5e5d3e0d2e842d04ec7778160ac564 https://doi.org/10.1111/j.1751-1097.1994.tb05016.x Zobrazit plný text záznamu Plný text