Sensing fatty acid binding protein with planar and fiber-optical surface plasmon resonance spectroscopy devices
| Autor: | Karl Cammann, Friedrich Spener, Reinhard Renneberg, Ulrich Kunz, Andreas Katerkamp |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Detection limit
Optical fiber Chemistry Calibration curve Surface plasmon Metals and Alloys Analytical chemistry Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention Transducer law Materials Chemistry lipids (amino acids peptides and proteins) Electrical and Electronic Engineering Surface plasmon resonance Spectroscopy Instrumentation Biosensor Biomedical engineering |
| Zdroj: | Sensors and Actuators B: Chemical. 32:149-155 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/0925-4005(96)80124-9 |
| Popis: | The human heart-type fatty acid binding protein (H-FABP) can be used as an early heart infarction marker in clinical diagnostics because its concentration in blood plasma increases about 1.5 to 3 hours after the onset of an acute myocardial infarction. Hence, it is of main interest to determine an infarct with a fast immunosensor. Surface plasmon resonance spectroscopy (SPRS) is one of the most versatile and sensitive direct optical technologies used for biochemical sensors. The aim of this study is the development of a direct optical immunosensor for the detection of H-FABP. Two different SPRS devices have been constructed: the well-known planar configuration and a new promising fiber-optical transducer. Both transducers are compared under the same conditions with regard to the measurement of H-FABP. They show a similar response and an excellent correspondence of the calibration curves. The detection limit of H-FABP in a competitive assay is about 200 ng ml −1 in both cases. |
| Databáze: | OpenAIRE |
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