A novel gold nanoparticle-based colorimetric assay for highly sensitive detection of ascorbic acid
Autor: | Anh-Tien Nguyen, Van Cuong Nguyen, Van Thuan Le, Thi Hong Anh Nguyen, Vu Tuyet Mai Nguyen, Yasser Vasseghian, Tan Phat Chau, Van-Dat Doan |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Materials Letters. 309:131307 |
ISSN: | 0167-577X |
DOI: | 10.1016/j.matlet.2021.131307 |
Popis: | In this study, a novel approach for colorimetric detection of ascorbic acid (AA) was proposed based on the peroxidase-like activity of gold nanoparticles, which were synthesized using Oldenlandia corymbosa leaves extract (OC-AuNPs). The OC-AuNPs synthesis was optimized at 0.5 mM Au3+, a reaction time of 20 min, and a temperature of 60 oC. The results showed that the biogenic OC-AuNPs presented as highly-efficient peroxidase mimics for catalyzing H2O2 to oxidize 3,3’,5,5’-tetramethylbenzidine forming a typical blue product with the characteristic absorption at 654 nm. The developed colorimetric method exhibited high sensitivity for AA with a detection limit of 0.26 μM in the linear range of 0 – 100 μM. The proposed assay can be serviced as a sensitive biosensor for the determination of AA in aqueous solutions due to its simple detection procedure, fast operation, and high sensitivity. |
Databáze: | OpenAIRE |
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