AAV Production Using Baculovirus Expression Vector System
| Autor: | Rachid Benchaouir, Karima Relizani, Quentin Sandro |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Baculovirus expression vector system Insect cell Computer science Process (engineering) viruses Genetic enhancement Gene transfer Context (language use) Computational biology law.invention 03 medical and health sciences 030104 developmental biology 0302 clinical medicine law 030220 oncology & carcinogenesis Recombinant DNA Production (economics) |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493990641 |
| DOI: | 10.1007/978-1-4939-9065-8_5 |
| Popis: | Gene transfer and gene therapy are powerful approaches for many biological research applications and promising avenues for the treatment of many genetic or cancer diseases. The most efficient gene transfer tools are currently derived from viruses. Among them, the recombinant adeno-associated viruses (AAVs) are vectors of choice for many fundamental and therapeutic applications. The increasing number of clinical trials involving AAVs demonstrates the need to implement production and purification processes to meet the quantitative and qualitative demands of regulatory agencies for the use of these vectors in clinical trials. In this context, the rise of production levels on an industrial scale appeared essential. The introduction, in 2002, of an AAV process using a baculovirus expression vector system (BEVS) has circumvented this technological lock. The advantage of BEVS in expanding the AAV production in insect cells has been to switch the process to bioreactor systems, which are the ideal equipment for scaling up. We describe here a method for producing AAV vectors using the BEVS which can be easily used by research laboratories wishing to overcome the difficulties associated with the scaling up of production levels. The method provides sufficient quantities of AAV vectors to initiate preclinical projects in large animal models or for research projects where a single batch of vectors will consolidate the repeatability and reproducibility of in vitro and especially in vivo experimental approaches. |
| Databáze: | OpenAIRE |
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