Proinsulin 2 Knockout NOD Mice
| Autor: | Danièle Dubois-Lafforgue, Jacques Jami, Laurent Mogenet, Christian Boitard, Patricia Krief, Karine Thébault |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
endocrine system
medicine.medical_specialty Type 1 diabetes endocrine system diseases Endocrinology Diabetes and Metabolism Insulin medicine.medical_treatment Nod Type 2 diabetes Biology medicine.disease Endocrinology Internal medicine Diabetes mellitus Internal Medicine medicine Allele Proinsulin NOD mice |
| Zdroj: | Diabetes. 51:S489-S493 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diabetes.51.2007.s489 |
| Popis: | Insulin is a major disease determinant in type 1 diabetes, type 2 diabetes, and related disorders. The role of variations in the expression of the insulin gene has been proposed in genetic susceptibility to the three pathological conditions in humans. In contrast to humans, rodents express two proinsulin isoforms. One isoform, proinsulin 1, is expressed exclusively in islets. The second, proinsulin 2, is expressed in islets and in other tissues, especially the thymus. We took advantage of the expression of these two isoforms to introduce a null proinsulin 2 allele in NOD mice and to evaluate the consequence of a variation of proinsulin 2 gene expression on the development of type 1 diabetes on the NOD genetic background. Heterozygote NOD mutant mice carrying a null proinsulin 2 mutation showed an increased incidence of type 1 diabetes at successive backcross generations. Plasma glucose and insulin levels were identical in prediabetic mutant and in wild-type mice at 4 weeks of age. Variation in insulin gene expression is hypothesized to interfere with diabetes development at both the islet and the thymus level. |
| Databáze: | OpenAIRE |
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