Assessing the effects of ultrasound parameters on P-selectin expression for active targeting of nanotherapeutics
| Autor: | Mark Burgess, Daniel Tylawsky, Quincey LaPlant, Daniel Heller |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | The Journal of the Acoustical Society of America. 153:A32-A32 |
| ISSN: | 1520-8524 0001-4966 |
| Popis: | P-selectin is a nanotherapeutic target for enhanced passage of nanoscale drug delivery systems across the vascular barrier and into tumors via transcytosis. While many tumors express P-selection, radiation can be used to induce expression in tumors devoid of P-selectin. This study aims to test whether ultrasound can elicit a similar response through mechanical or thermal bioeffects. Preliminary experiments were performed in vitro using brain tissue derived mouse endothelial cells. A Vevo SoniGene system was used to sonicate cells with low-intensity (2 W/cm2) pulsed ultrasound in a 12-well cell culture plate. Upregulation of P-selectin was seen using a 60 second treatment duration at 50% and 100% duty cycles via flow cytometry analysis. The mean fluorescence intensity, normalized to nontreated cells, was 32 + /- 17.4 and 103 + /- 37.2 for 50% and 100% duty cycles, respectively. Viability remained high with 72.6 + /- 14.5 and 77.5 + /- 8.3% of cells viable for both 50% and 100% duty cycle groups, respectively. These results highlight the potential of ultrasound for active targeting using non-ionizing energy and for tumors where radiation is not feasible. Future studies will identify associated bioeffects and compare ultrasound with radiation for nanotherapeutic delivery in small animal tumor models. |
| Databáze: | OpenAIRE |
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