An in vitro kinetic study of the mixed inhibition of honeybee hemolymph PNP-α-D-glucosidase by sucrose

Autor:	M. R. J. Morgan, M. Bounias
Rok vydání:	1981
Předmět:	Pharmacology Sucrose Mixed inhibition Cell Biology Biology Inhibitory postsynaptic potential Trehalose In vitro Cellular and Molecular Neuroscience chemistry.chemical_compound chemistry Biochemistry Hemolymph Molecular Medicine Molecular Biology
Zdroj:	Experientia. 37:1147-1149
ISSN:	1420-9071 0014-4754
DOI:	10.1007/bf01989887
Popis:	Sucrose acts in vitro as a mixed inhibitor of (V+K+n) type towards honeybee hemolymph PNP-α-D-glucosidase activity. Between the stages of emergence and foraging, there is little change in the effect of the inhibitor on VM (fi=from 1.31 to 1.35 respectively) or n (fi=from 1.09 to 1.07) but K is more markedly affected (fi−1=from 1.17 to 1.87)M. These observations reflect the decrease of Ki from 277 to 98 mM and of I50 from 154 to 111 mM, but K′i scarcely alters during development (from 477 to 425 mM). These inhibitory effects of sucrose are intermediate between those previously reported for trehalose and glucose.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0ea6c11fdbd4a0d3a00a7dce45ae7c54 https://doi.org/10.1007/bf01989887 Zobrazit plný text záznamu Full text from SpringerLink