Development of Mongolian Gerbil 1-cell Embryos to Blastocysts in Co-culture with Oviductal Cells in TCM 199 Supplemented with Pyruvate, Lactate and Fetal Calf Serum
| Autor: | Takeshi Saruwatari, Koh-ichi Hamano, Hirotada Tsujii |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Fetus animal structures animal diseases Cell Embryo culture Embryo Biology Gerbil In vitro Andrology Endocrinology medicine.anatomical_structure In vivo Internal medicine parasitic diseases embryonic structures medicine Animal Science and Zoology sense organs Pyruvate lactate |
| Zdroj: | Journal of Reproduction and Development. 48:287-292 |
| ISSN: | 1348-4400 0916-8818 |
| DOI: | 10.1262/jrd.48.287 |
| Popis: | To develop an in vitro culture system for Mongolian gerbil embryos, we examined the effect of co-culture with oviductal epithelial cells on the development of 1- and 2-cell embryos collected from superovulated gerbils. In the first experiment, 2-cell embryos were cultured in modified TCM 199 supplemented with pyruvate, lactate and fetal calf serum (mTCM199) with or without gerbil or mouse oviductal cells. In the second experiment, in 1-cell embryos were cultured with or without gerbil oviductal cells in mTCM199. Although, 2-cell gerbil embryos did not develop beyond the 16-cell stage without oviductal cells in both TCM199 and mTCM199, co-culture with gerbil or mouse oviductal cells supported the development of 2-cell gerbil embryos to blastocysts. Co-culture with gerbil oviductal cells in mTCM199, but not in TCM199, also supported the development of 1-cell embryos to blastocysts. There were no differences between the in vivo and in vitro developed blastocysts in morphological appearance and cell numbers. This co-culture system can be used for in vitro culture of Mongolian gerbil embryos. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|