Antioxidant activities and liquid chromatography with electrospray ionization tandem mass spectrometry characterization and quantification of the polyphenolic contents ofRumex nervosusVahl leaves and stems
| Autor: | Won Sup Lee, Ho-Chul Shin, Kebede Taye Desta, Gon-Sup Kim, Yun-Hi Kim, Mohamad Warda, A. M. Abd El-Aty, Sung Joong Lee, Soo Taek Kim, Soo-Jung Lee, Jae-Han Shim, Sung Chul Shin |
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| Rok vydání: | 2016 |
| Předmět: |
Orientin
chemistry.chemical_classification Chromatography 010405 organic chemistry Chemistry Electrospray ionization 010401 analytical chemistry Filtration and Separation Catechin Hydroxycinnamic acid Tandem mass spectrometry 01 natural sciences 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound Chlorogenic acid Polyphenol Caffeic acid |
| Zdroj: | Journal of Separation Science. 39:1433-1441 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.201600018 |
| Popis: | In the present study, four compounds, viz. chlorogenic acid, catechin, orientin, and apigenin-O-acetylglycoside among 18 polyphenol compounds (17 flavonoids and one hydroxycinnamic acid derivative) were characterized for the first time in Rumex nervosus leaves and stems by using liquid chromatography with electrospray ionization tandem mass spectrometry. Method validation in terms of determination coefficient, limits of detection, and quantification were ≥ 0.9979, 0.68-1.61, and 2.27-5.38 mg/L, respectively. Accuracy, expressed as percent recovery for two spiking levels (10 and 50 mg/L), were in the range 78.9-110.6% with the exception of caffeic acid. The relative standard deviations were 1-17%. The total polyphenol content was higher by approximately two times in the leaf (1073 mg/kg fresh sample) than in the stem (519.86 mg/kg fresh sample). The antioxidant effects increased in a dose-dependent manner, and the scavenging activities, investigated by measuring 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity, ferrous ions chelating activity, superoxide anion radical scavenging activity, and ferric reducing antioxidant power activity, were significant (p < 0.05) using low concentrations of the leaf extract. Overall, the present study suggests that different parts of R. nervosus have great potential for producing a range of extracts with potential applications in medicine. |
| Databáze: | OpenAIRE |
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