Engineering pyridoxal kinase PdxY-integrated Escherichia coli strain and optimization for high-level 5-aminolevulinic acid production
| Autor: | I-Son Ng, Tzu-Hsuan Yu, Chengfeng Xue |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Rhodobacter biology General Chemical Engineering 02 engineering and technology General Chemistry Tricarboxylic acid 010402 general chemistry 021001 nanoscience & nanotechnology medicine.disease_cause biology.organism_classification 01 natural sciences Pyridoxal kinase 0104 chemical sciences Citric acid cycle chemistry.chemical_compound chemistry Biochemistry Glycine medicine Fermentation 0210 nano-technology Escherichia coli Pyridoxal |
| Zdroj: | Journal of the Taiwan Institute of Chemical Engineers. 120:49-58 |
| ISSN: | 1876-1070 |
| DOI: | 10.1016/j.jtice.2021.03.017 |
| Popis: | Recently, 5-aminolevulinic acid (ALA) has gained significant attention in research due to its extensive applications in agriculture, aquaculture, cosmetics, and photodynamic therapy for cancer. Microbial production of ALA is a green, high-efficient, and sustainable process. Herein, we have engineered a new strain of Escherichia coli called P dxY I ntegrative E . c oli E nhancer (PIECE). In this strain, the super salvage pathway was fine-tuned to enhance the regeneration of pyridoxal 5′-phosphate (PLP), on which the activity of ALA synthase (ALAS) is highly dependent. The PIECE strain, harboring ALA synthase (ALAS) from Rhodobacter capsulatus, produced 1.99 g/L ALA and 5.15 g/L biomass, that was a 4.33-fold increase in ALA production and a 1.84-fold increase in biomass compared to the parent strain. The metabolic flux analysis indicated that PIECE would redirect more carbon into the tricarboxylic acid (TCA) cycle and generate more glycine and glutamate, thereby increasing the ALA and biomass. Finally, the maximum ALA yield of 8.21 g/L and a productivity of 0.228 g/L/h were obtained by using fed-batch fermentation. The results showed that PIECE was not only feasible for high-level ALA production but also had significant potential to reprogram the carbon flux in the TCA cycle to produce value-added chemicals in the future. |
| Databáze: | OpenAIRE |
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