Study of Polyphenol Components in Extracts of Arctic Brown Algae of Fucus vesiculosus Type by Liquid Chromatography and Mass-Spectrometry
| Autor: | Andrey Stavrianidi, Polina Turova, P. A. Kaplitsin, I. I. Pikovskoi, A. S. Druzhinina, D. V. Ovchinnikov, O. Yu. Khoroshev, Bogolitsyn Konstantin G, Oleg A. Shpigun, Anastasia Parshina |
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| Rok vydání: | 2020 |
| Předmět: |
Chromatography
biology Chemistry 010401 analytical chemistry Extraction (chemistry) Fucus vesiculosus Fractionation 010402 general chemistry biology.organism_classification Mass spectrometry 01 natural sciences High-performance liquid chromatography 0104 chemical sciences Analytical Chemistry Brown algae Column chromatography Polyphenol |
| Zdroj: | Journal of Analytical Chemistry. 75:633-639 |
| ISSN: | 1608-3199 1061-9348 |
| Popis: | The extraction of polyphenol component of arctic brown algae of the Fucus vesiculosus type and the subsequent fractionation of the extract by column chromatography on a Sephadex LH-20 adsorbent are carried out. The obtained fractions are characterized by size-exclusion chromatography, HPLC with UV and mass spectrometric detection (HPLC–UV-MS), and matrix-assisted laser desorption/ionization mass spectrometry (MALDI–MS). Using the MALDI–MS method, the presence of oligomeric compounds containing up to 13 phloroglucin structural units is detected. Using HPLC–UV-MS, oligomeric compounds in the mass range 374–870 Da (trimers–octamers) are detected, and their ratio is estimated. |
| Databáze: | OpenAIRE |
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