Expression of genes encoding xylose isomerases from and in the methylotrophic yeast

Autor:	Andriy Y. Voronovsky, Olena B. Ryabova, Olena V. Verba, Olena P. Ishchuk, Kostyantyn V. Dmytruk, Andriy A. Sibirny
Rok vydání:	2005
Předmět:	Xylose isomerase Streptomyces coelicolor General Medicine Isomerase Xylose Biology biology.organism_classification medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Streptomyces Yeast chemistry.chemical_compound chemistry Biochemistry medicine Fermentation Escherichia coli
Zdroj:	FEMS Yeast Research. 5:1055-1062
ISSN:	1567-1356
DOI:	10.1016/j.femsyr.2005.09.001
Popis:	The thermotolerant methylotrophic yeast Hansenula polymorpha is able to ferment xylose to ethanol at high temperatures. H. polymorpha xylose reductase and xylitol dehydrogenase are involved during the first steps of this fermentation. In this article, expression of bacterial xylA genes coding for xylose isomerases from Escherichia coli or Streptomyces coelicolor in the yeast H. polymorpha was shown. The expression was achieved by integration of the xylA genes driven by the promoter of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase gene ( HpGAP) into the H. polymorpha genome. Expression of the bacterial xylose isomerase genes restored the ability of the H. polymorpha Δxyl1 mutant to grow in a medium with xylose as the sole carbon source. This mutant has a deletion of the XYL1 gene encoding xylose reductase and is not able to grow in the xylose medium. The H. polymorpha Δxyl1(xylA) transformants displayed xylose isomerase activities, which were near 20% of that of the bacterial host strain. The transformants did not differ from the yeast wild-type strain with respect to ethanol production in xylose medium.
Databáze:	OpenAIRE
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