| Autor: |
Joseph K. Welply, Broschat K, Jeffery L. Keene, Peter Richard Scudder, S Z Abbas, Christina N. Steininger, Gary S. Jacob, Susan C. Howard, C Kirmaier, Jon J. Schmuke |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Advances in Experimental Medicine and Biology ISBN: 9781461357681 |
| DOI: |
10.1007/978-1-4615-1885-3_31 |
| Popis: |
Recruitment of neutrophils to sites of inflammation is now believed to occur through an initial rolling interaction at the luminal surface of activated endothelium and is mediated by a class of mammalian lectins referred to as the selectins. Selectins recognize carbohydrate determinants on co-receptors. It is generally believed that many selectin molecules must bind to many carbohydrate receptor molecules i.e. multivalent binding, to enable sufficient binding strength to elicit the rolling response between the neutrophil and the endothelial cell. One of the approaches to the generation of more potent molecular antagonists of the selectin-mediated cell-cell interaction is to mimic the multivalent interaction in a single compound. Recent experiments utilising conjugated forms of sialyl Lewisx-BSA have explored this feasibility (Welply et al., 1994). In that study, monovalent sLex (sialic acidα2-3Galβ1-4(Fucαl-3)GlcNAc), the minimum binding determinant for E-selectin, as well as monovalent sialyllactosamine (sialic acidα2-3Galβ1-4GlcNAc), a non-binding structure, and the corresponding multivalent BSA-conjugated forms were tested for their ability to inhibit binding of HL-60 cells to immobilised E-selectin. As expected, only sLex and sLex-BSA were found to do so. sLex 16-BSA (16 mol tetrasaccharide/mol BSA) showed a dose-dependent inhibition of HL-60 binding with a measured IC50 of 1μM; demonstrating close to a three-order of magnitude enhancement of inhibitory activity compared to free sLex. This result indicated that multivalent forms of sLex are capable of binding to E-selectin with higher affinity than do monovalent glycans. In another study, fluorescent forms of monovalent sLex were synthesized and used to measure a true thermodynamic dissociation constant for the monovalent sLex:E-selectin interaction of 120±31 μM. (Jacob et.al., 1995). |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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