Lactobacillus kefiranofaciens and Lactobacillus satsumensis isolated from Brazilian kefir grains produce alpha-glucans that are potentially suitable for food applications
| Autor: | Igor M. Paiva, Elisabeth Neumann, Jacques Robert Nicoli, R.S. Steinberg, Maria José Valenzuela Bell, Elaine M. Souza-Fagundes, Ivana Silva Lula, Thiago de Oliveira Mendes, Álvaro Cantini Nunes |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
food.ingredient ved/biology.organism_classification_rank.species Polysaccharide 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology food Lactobacillus Food science Sugar Glucan chemistry.chemical_classification biology ved/biology Food additive Kefir 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Lactic acid 030104 developmental biology Biochemistry chemistry Food Science Lactobacillus kefiranofaciens |
| Zdroj: | LWT - Food Science and Technology. 72:390-398 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2016.05.010 |
| Popis: | Lactic acid bacteria produce exopolysaccharides, which have broad application in the food industry, especially as thickeners, stabilizers, and gelling agents. Additionally, some biopolymers have been regarded as health promoters due to their role as prebiotics and their immunomodulatory properties. This study aimed to characterize three exopolysaccharides produced by novel Lactobacillus strains from Brazilian milk or sugar water kefir and to determine their biocompatibility. Lactobacillus kefiranofaciens 1P3 and Lactobacillus satsumensis 10P and 10P2 were grown in the presence of sucrose, and their exopolysaccharides were purified and structurally elucidated. Glucose was the only sugar found in the polysaccharide chains, so most of the glucose residues were linked by α-(1,6) glycosidic bonds (∼90%) and the estimated molecular weight was 800 kDa. According to the NIR spectra, α-glucan 1P3, 10P, and 10P2 were all different compounds. The in vitro cytotoxicity of the polymers was low or nonexistent, especially in nontumor cells. The polymers had no significant effects on the expression of proinflammatory and regulatory cytokines in the intestine of mice. In contrast, the intestinal IgA + B cells were significantly higher. Taken together, this study suggests that these exopolysaccharides might be useful as food additives due to their safety features to the host. |
| Databáze: | OpenAIRE |
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