Formation of blends of various poly(3-hydroxyalkanoic acids) by a recombinant strain of Pseudomonas oleovorans
| Autor: | Alexander Steinbüchel, Arnulf Timm, David Byrom |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences Chloroform biology 030306 microbiology Chemistry General Medicine Polymer Pseudomonas oleovorans biology.organism_classification Applied Microbiology and Biotechnology Copolyester Polyester Gel permeation chromatography 03 medical and health sciences chemistry.chemical_compound Organic chemistry Ammonium Fermentation 030304 developmental biology Biotechnology Nuclear chemistry |
| Zdroj: | Applied Microbiology and Biotechnology. 33:296-301 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/bf00164525 |
| Popis: | Recombinant strains of Pseudomonas oleovorans, which harbour the poly(3-hydroxybutyrate)-biosynthetic genes of Alcaligenes eutrophus, accumulated poly(hydroxyalkanoates), composed of 3-hydroxybutyrate(3HB), 3-hydroxyhexanoate (3HHx) and 3-hydroxyactanoate (3HO), up to 70% of the cell dry weight if the cells were cultivated with sodium octanoate as the carbon source. Physiological and chemical analysis revealed multiple evidence that this polymer is a blend of the homopolyester poly(3HB) and of the copolyester poly(3HHx-co-3HO) rather than a random or a block copolyester of 3HB, 3HHx and 3HO. The molar ratio between poly(3HHx-co-3HO) and poly(3HB) varied drastically during the process of fermentation. Whereas synthesis of poly(3HHx-co-3HO) started immediately after ammonium was exhausted in the medium, synthesis of poly(3HB) occurred only after a lag-phase. From freeze-dried cells poly(3HHx-co-3HO) was much more readily extracted with chloroform than was poly(3HB). The blend was fractionated into petrol-ether-insoluble poly(3HB) and petrol-ether-soluble poly(3HHx-co-3HO). The molecular weight values of these polyesters measured by gel permeation chromatography were 2.96 × 106 and 0.35 × 106 and were similar of those polymers accumulated by A. eutrophus or by wild-type P. oleovorans, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink