Genome-Wide Association Study on Resistance to Rice Black-Streaked Dwarf Disease Caused by Rice black-streaked dwarf virus
Autor: | Guofang Lan, Chen Kai, Liu Qing, Jianlong Xu, Zhikang Li, Fan Zhang, Xiuqin Zhao, Yajun Zhu, Congcong Shen |
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Rok vydání: | 2021 |
Předmět: |
0106 biological sciences
0301 basic medicine Genetics Candidate gene Haplotype food and beverages Genome-wide association study Single-nucleotide polymorphism Plant Science Biology biology.organism_classification 01 natural sciences Virus Japonica 03 medical and health sciences 030104 developmental biology Rice black-streaked dwarf virus Agronomy and Crop Science Gene 010606 plant biology & botany |
Zdroj: | Plant Disease. 105:607-615 |
ISSN: | 1943-7692 0191-2917 |
Popis: | Rice black-streaked dwarf disease caused by Rice black-streaked dwarf virus (RBSDV) is one of the most destructive viral diseases of rice. Thus, it is imperative that resistant rice germplasms are screened for novel RBSDV-resistant genes. RBSDV resistance of a diverse global collection comprising 1,953 rice accessions was evaluated under natural conditions across 3 years. The average disease incidences of the Xian/indica (XI) subgroup were significantly lower than those of the Geng/japonica (GJ) subgroup. Interestingly, most XI-1A accessions in the Xian subgroup were significantly more susceptible than XI-1B accessions, even though XI-1A and XI-1B have a close phylogenetic relationship. Four Xian accessions stably and highly resistant to RBSDV were consistently identified in 2 years. Ten genomic regions (GRs) with 147 single nucleotide polymorphisms associated with RBSDV resistance were detected by a single-locus genome-wide association study (GWAS), of which five were repeatedly identified in a multilocus GWAS. Two previously reported GRs, grRBSDV-6.1 and grRBSDV-6.3, which were repeatedly detected as stably and highly associated with RBSDV resistance, contained 17 and seven genes, respectively, with significant differences of resistance among haplotypes. Haplotype analyses of the candidate genes LOC_Os06g03150 in grRBSDV-6.1 and LOC_Os06g31190 in grRBSDV-6.3 suggested that the former gene is mainly associated with the differentiation of resistance within the Xian subgroup and the latter gene mainly explains the difference in the resistance between Xian and Geng. Another three novel resistance GRs (grRBSDV-1.1, grRBSDV-7.1, and grRBSDV-9.1) were identified. Our findings may enhance the application of disease-resistant rice germplasms for breeding RBSDV-resistant varieties. |
Databáze: | OpenAIRE |
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