Isocratic Separation of Fatty Acid Derivatives by Reversed Phase Liquid Chromatography. Influence of the Solvent on Selectivity and Rules for Elution Order
| Autor: | S. V. Coen, J. P. Roggero |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Solvent
chemistry.chemical_classification Chromatography Resolution (mass spectrometry) Chemistry Elution Hydrophilic interaction chromatography Molecular Medicine Fatty acid lipids (amino acids peptides and proteins) Thermoresponsive polymers in chromatography Reversed-phase chromatography Selectivity |
| Zdroj: | Journal of Liquid Chromatography. 4:1817-1829 |
| ISSN: | 0148-3919 |
| DOI: | 10.1080/01483918108064849 |
| Popis: | The p-bromophenacyl esters of 16 fatty acids (C12-C22) have been separated by isocratic chromatography on a Radial Pak A cartridge (Reverse phase C18 material). The separation factors α were measured using two solvent mixtures of comparable strength and the superiority of methanol-water to acetonitrile-water becomes evident. Five precise rules are established, which indicates the retention of every fatty acid. They explain the chromatographic process i.e. elution order, resolution and selectivity. |
| Databáze: | OpenAIRE |
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