Enzymatic hydrolysis of thermally pre‐treated chitin and antimicrobial activity of N , N ′‐diacetylchitobiose
| Autor: | Keshavan Niranjan, Andreas K Karatzas, Constantina Kourmentza, Munira Zainal Abidin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
General Chemical Engineering
02 engineering and technology 010501 environmental sciences 01 natural sciences Autoclave Inorganic Chemistry Hydrolysis chemistry.chemical_compound Chitin Enzymatic hydrolysis Waste Management and Disposal 0105 earth and related environmental sciences Chromatography biology Renewable Energy Sustainability and the Environment Organic Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Antimicrobial Pollution Fuel Technology chemistry Chitinase biology.protein 0210 nano-technology Streptomyces griseus Sodium acetate Biotechnology |
| Zdroj: | Journal of Chemical Technology & Biotechnology. 94:2529-2536 |
| ISSN: | 1097-4660 0268-2575 |
| DOI: | 10.1002/jctb.6043 |
| Popis: | BACKGROUND: N,N′‐diacetylchitobiose (GlcNAc₂) is known to be highly functional and offers a wide range of applications, especially as an antimicrobial agent. In this study, a thermal pre‐treatment process using steam under pressure in an autoclave was employed to facilitate subsequent enzymatic hydrolysis of chitin with chitinase from Streptomyces griseus. RESULTS: Pre‐treatment of chitin with 0.05 mol L⁻¹ sodium acetate buffer (pH = 6.0) at 121 °C for 60 min, followed by enzymatic hydrolysis involving 24 h incubation, were found to be the best conditions for producing GlcNAc₂. The GlcNAc₂ thus obtained was tested regarding its antimicrobial activity against Gram‐negative and Gram‐positive strains and showed minimum inhibitory concentrations at 5 and 10% (w/v) against Escherichia coli K‐12 and Listeria monocytogenes 10403S, respectively. CONCLUSIONS: The extent of swelling and crystallite size of chitin increased with pre‐treatment residence time, and enhanced the rate of subsequent hydrolysis using chitinase. © 2019 Society of Chemical Industry |
| Databáze: | OpenAIRE |
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