Evaluation of an Automated Equilibration Technique for Deuterium/Hydrogen Isotope Ratio Measurements with Respect to Assessing Total Energy Expenditure by the Doubly Labelled Water Method

Autor:	Frank Thielecke, Rudolf Noack
Rok vydání:	1997
Předmět:	Investigation methods Energy expenditure Total energy expenditure Deuterium Chemistry Hydrogen isotope Analytical chemistry Energy metabolism Spectroscopy Biological fluid Dilution
Zdroj:	Journal of Mass Spectrometry. 32:323-327
ISSN:	1096-9888 1076-5174
DOI:	10.1002/(sici)1096-9888(199703)32:3<323::aid-jms491>3.0.co;2-x
Popis:	The doubly labelled water (DLW) method for measuring energy expenditure in free living subjects suffers from inaccuracy and imprecision mainly owing to analytical problems An automated H/D/O equilibration device was tested for precision, internal validity and matrix effects with particular consideration of DLW studies. The precision was found to be 0.5‰ and 0.04‰ for 2 H and 18 O, respectively. Dilution series with water standards and urine showed a high linearity and no matrix effects. In addition, the automated preparation is less laborious and time consuming and reduces sources of analytical imprecisions in studies measuring energy expenditure by the DLW method.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0e21b127262e06cab66bec93654403be https://doi.org/10.1002/(sici)1096-9888(199703)32:3<323::aid-jms491>3.0.co Zobrazit plný text záznamu Plný text