Saline stress modulates mir‐27a, mir‐103 and mir135 in 3T3‐PKD‐1 cells (802.15)

Autor: M. Eugenia Torres-Márquez, Jim Sinnett-Smith, Ruth Ruiz Esparza-Garrido, Enrique Rozengurt
Rok vydání: 2014
Předmět:
Zdroj: The FASEB Journal. 28
ISSN: 1530-6860
0892-6638
Popis: PKD is a founding member of CAMK group of kinases and there is ample evidence of its participation in important cellular process including stress. Due to the lack of specific inhibitors for its action in vivo, its protein targets are scarcely described. Even less is known about its role at regulating the transcription of target proteins. The miRNA are important regulators of protein expression and several groups are currently undertaking studies about its targets, enriching a database that will enable a better understanding of the networks in which they participate. In this work we aimed to identify miRNA that are modulated by hyperosmotic stress in 3T3 expressing PKD-1 cells as a means to understand the PKD participation in this cellular process. We obtained the RNA from 3T3-PKD-1 cells and their control counterparts, without or with an hour challenge with 0.3M NaCl, and sought to identify the up- or down- regulated miRNA. This was performed using miRNA3.0 Affymetrix microchips, and analyzed with softwar...
Databáze: OpenAIRE
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