Elimination of Interfering Chromogens in the Zimmerman Reaction for Measuring 17-Ketosteroids

Autor:	Arnold G. Ware, Fred Cox, Clifford B. Walberg, Ronald L. Searcy, Solomon Notrica, James A. Demetriou
Rok vydání:	1959
Předmět:	Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Analytical chemistry Aqueous ethanol
Zdroj:	Clinical Chemistry. 5:479-487
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/5.5.479
Popis:	A simple extraction technic is described for separating interfering chromogens from the 17-KS complex formed when the Zimmerman reaction is applied to urinary extracts. The procedure is performed immediately after the Zimmerman reaction is completed. It allows quantitation of the steroid present in a filter photometer at one wave length. Results compare favorably with those obtained after dilution of the Zimmerman complex with 70% aqueous ethanol and application of Allen's correction.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0e10f2f89d863bb3d5a9740eff1f7e94 https://doi.org/10.1093/clinchem/5.5.479 Zobrazit plný text záznamu