Identification of Puffer Fish of the GenusLagocephalus: L. lunaris, L. spadiceusandL. inermis, Using Multiplex PCR
| Autor: | Danai Sangthong, Pradit Sangthong, Lertluk Ngernsiri |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lagocephalus
Genetics Mitochondrial DNA biology Cytochrome b biology.organism_classification MT-RNR1 Fish products Applied Microbiology and Biotechnology Molecular biology chemistry.chemical_compound chemistry Multiplex polymerase chain reaction Primer (molecular biology) DNA Food Science Biotechnology |
| Zdroj: | Food Biotechnology. 28:216-231 |
| ISSN: | 1532-4249 0890-5436 |
| DOI: | 10.1080/08905436.2014.931865 |
| Popis: | A multiplex PCR assay was developed to identify widespread poison-containing puffer fishes of the genus Lagocephalus: L. lunaris, L. spadiceus and L. inermis, using a novel KUGEN_ILSspec primer set which is specific for 12S ribosomal RNA (12S rRNA) and Cytochrome b (Cyt b) genes. KUGEN_ILSspec set is composed of fish positive-amplified primers that produced a 289-bp fragment while L. lunaris-, L. spadiceus- and L. inermis-specific primers produced 123-, 196- and 493-bp fragments, respectively. The sensitivity of this primer set was found to be high as it was capable of amplifying targeted DNA at concentrations as low as 1 ng/μL. Moreover, the primer set was shown to amplify intact DNA and species-specific fragments from heat-processed food. Consequently, multiplex PCR technique in combination with KUGEN_ILSspec primer set could offer an effective measure for detecting poisonous puffer fish contamination in both fresh and processed fish products, which will greatly aid in public health control and law enfo... |
| Databáze: | OpenAIRE |
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