Segregation of Acute Leptin and Insulin Effects in Distinct Populations of Arcuate Proopiomelanocortin Neurons
| Autor: | Kevin W. Williams, Carol F. Elias, Joel K. Elmquist, Syann Lee, Charlotte E. Lee, Michelle J. Choi, Michael Scott, Lisandra O. Margatho |
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| Rok vydání: | 2010 |
| Předmět: |
endocrine system
medicine.medical_specialty Leptin receptor biology General Neuroscience Leptin Insulin medicine.medical_treatment digestive oral and skin physiology In situ hybridization Insulin receptor Endocrinology nervous system Proopiomelanocortin Hypothalamus Internal medicine medicine biology.protein hormones hormone substitutes and hormone antagonists PI3K/AKT/mTOR pathway |
| Zdroj: | The Journal of Neuroscience. 30:2472-2479 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Acute leptin administration results in a depolarization and concomitant increase in the firing rate of a subpopulation of arcuate proopiomelanocortin (POMC) cells. This rapid activation of POMC cells has been implicated as a cellular correlate of leptin effects on energy balance. In contrast to leptin, insulin inhibits the activity of some POMC neurons. Several studies have described a “cross talk” between leptin and insulin within the mediobasal hypothalamus via the intracellular enzyme, phosphoinositol-3-kinase (PI3K). Interestingly, both insulin and leptin regulate POMC cellular activity by activation of PI3K; however, it is unclear whether leptin and insulin effects are observed in similar or distinct populations of POMC cells. We therefore used dual label immunohistochemistry/in situhybridization and whole-cell patch-clamp electrophysiology to map insulin and leptin responsive arcuate POMC neurons. Leptin-induced Fos activity within arcuate POMC neurons was localized separate from POMC neurons that express insulin receptor. Moreover, acute responses to leptin and insulin were largely segregated in distinct subpopulations of POMC cells. Collectively, these data suggest that cross talk between leptin and insulin occurs within a network of cells rather than within individual POMC neurons. |
| Databáze: | OpenAIRE |
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