Agrobacterium-mediated transformation of Bangladesh indica for conferring salt tolerance

Autor: Zeba I. Seraj, M. Bakhtiar Hossain, Glenn B. Gregorio, Hasina Akhter, M. A. Salam, Noorain M. Rasul, Haseena Khan, Shamim Hossain
Rok vydání: 2002
Předmět:
Zdroj: Tasks for vegetation science ISBN: 9789048160198
DOI: 10.1007/978-94-017-0067-2_18
Popis: In the Bangladesh coast, 1 million hectares of cultivable land is affected by considerable salinity. The larger portions exist in the western coast and smaller areas in the eastern coast up to the southern tip of Bangladesh in Cox’s Bazar. There is a general lack of MV varieties suitable for the above regions. There are two approaches for the production of suitable rice varieties-establishment of genetic markers with the help of IRRI to aid and accelerate breeding programs and establishment of Agrobacterium-mediated transformation techniques with the goal of incorporation of genes for the production of osmoprotective compounds. Rice does not produce osmoprotective compounds; therefore if we transform moderately salt tolerant varieties using above strategy, it is expected that there will be a further enhancement of salt tolerance. We have acquired the genes, mannitol-1-phosphate dehydrogenase from IRRI for the production of mannitol and trehalose synthase and phosphatase from Myongji University, Korea, for the production of trehalose. Transformation experiments using the two above genes are in progress with BR5331, an advanced T. Aman moderately salt tolerant rice variety. Other varieties being used are farmer-popular BR28 and BR29 and IRRT salt tolerant variety 1R51500. The methods of transformation that are being used are those that were previously established with rice varieties that were shown to be easily transformed using marker genes.
Databáze: OpenAIRE