Autor: M. Narasimha Rao, C. Ayyanna, B. V.V. Ratnam, M. Damodar Rao, S. Subba Rao
Rok vydání: 2003
Předmět:
Zdroj: World Journal of Microbiology and Biotechnology. 19:523-526
ISSN: 0959-3993
Popis: The quantitative effects of temperature, pH and time of fermentation were investigated on simultaneous saccharification and fermentation (SSF) of ethanol from sago starch with glucoamylase (AMG) and Zymomonas mobilis ZM4 using a Box–Wilson central composite design protocol. The SSF process was studied using free enzyme and free cells and it was found that with sago starch, maximum ethanol concentration of 70.68 g/l was obtained using a starch concentration of 140 g/l, which represents an ethanol yield of 97.08%. The optimum conditions for the above yield were found to be a temperature of 36.74 °C, pH of 5.02 and time of fermentation of 17 h. Thus by using the central composite design, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs.
Databáze: OpenAIRE