Enhanced hydration stability of Bombyx mori silk fibroin/PEG 600 composite scaffolds for tissue engineering

Autor:	Chiara G.M. Gennari, Pankaj Vagdama, Francesco Cilurzo, L.A. Marotta, Luisa Montanari, Paola Minghetti, Francesca Selmin, Barbara Viviani
Rok vydání:	2014
Předmět:	Materials science Polymers and Plastics biology fungi Composite number Fibroin Adhesion biology.organism_classification Chemical engineering Tissue engineering Bombyx mori Reagent Polymer chemistry PEG ratio Protein adsorption
Zdroj:	Polymers for Advanced Technologies. 25:532-538
ISSN:	1042-7147
DOI:	10.1002/pat.3282
Popis:	Silk fibroin scaffolds obtained via regeneration by Ajisawa's reagent (CaCl2/H2O/EtOH, mole ratio 1:8:2) and freeze-drying followed by steam sterilization resulted unstable when incubated in physiological medium over 1-month period since the oxygen diffusivity and mechanical resistance to compression significantly decreased. Blending of regenerated silk fibroin with PEG 600 lead to a novel scaffold with an improved water stability. Multi-parameter characterization has revealed that scaffolds made by composite material were stable in physiological media over at least 1 month of incubation. Interestingly, the presence of PEG has a notable impact on wettability and protein adsorption. This increase in hydrophilicity could be beneficial for initial cell adhesion and survival as well as for the adsorptive loading of growth factors. Copyright © 2014 John Wiley & Sons, Ltd.
Databáze:	OpenAIRE
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