| Autor: |
Vijayendra Dasari, Lisa McNeil, Kirrilee Beckett, Matthew Solomon, George Ambalathingal, Martin Steinbuck, Aniela Jakubowski, Lochana Seenappa, Erica Palmer, Jeff Zhang, Chris Haqq, Peter DeMuth, Rajiv Khanna |
| Rok vydání: |
2022 |
| Popis: |
Recent emergence of a causal like between Epstein-Barr virus (EBV) and multiple sclerosis has generated considerable interest in the development of an effective vaccine against EBV. Here we describe a novel vaccine formulation based on a lymph node targeting Amphiphile (AMP) vaccine adjuvant, AMP-CpG, admixed with EBV gp350 glycoprotein and a novel EBV-polyepitope protein (EBVpoly) that includes 20 CD8+ T-cell epitopes from EBV latent and lytic antigens. Potent gp350-specific IgG responses were induced in mice with titers > 100,000 in AMP-CpG vaccinated mice. Immunization including AMP-CpG also induced high frequencies of polyfunctional gp350-specific CD4+ T-cells and EBVpoly-specific CD8+ T-cells that were 2-fold greater than soluble CpG and were maintained for > 7 months post immunization. This combination of broad humoral and cellular immunity against multiple viral determinants is likely to provide better protection against primary infection and control of latently infected B cells leading to protection against the development of EBV-associated diseases. Teaser: A lymph node targeted AMP-CpG subunit EBV vaccine induces potent and durable immunity in HLA expressing mice. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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