| Autor: |
Ramana Terli, Siva Kumar Kandula |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Journal of Pharmacy Research. 7:397-403 |
| ISSN: |
0974-6943 |
| DOI: |
10.1016/j.jopr.2013.04.047 |
| Popis: |
Background An indigenous marine Streptomyces coeruleorubidus BTSS-301 has been isolated from marine sediment sample near Visakhapatnam coast at a depth of 30 m. It exhibited broad spectrum of antimicrobial activity. However, for maximum production of a specific bioactive compound from indigenous isolates medium formulation is an essential step. Methods Optimization experiments for various carbon and nitrogen sources and cultural parameters were performed by shake flask culture method. The active compound in culture filtrate was extracted into ethyl acetate and then purified by silica gel column chromatography. The purified active compound was characterized by spectroscopy studies. The minimum inhibitory concentration was determined by broth dilution method. Results & discussion Optimization studies revealed that the highest antibiotic production was obtained when glucose at 10.0 g/l and NH4NO3 at 2.5 g/l were used as carbon and nitrogen sources respectively. Cultural parameters revealed that 96 h of incubation at 30 °C at 180 rpm with pH 7.2 of the production medium exhibited maximum yield. The purified active compound is identified as N-ethyl-2-(2-(3-hydroxybutyl) phenoxy) acetamide. Conclusion The antimicrobial compound from S. coeruleorubidus BTSS-301 could be a candidate in the generation of new antimicrobial agents from marine Streptomyces. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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