A Metabolomics-Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures
| Autor: | Rein V. Ulijn, Karl Burgess, Matthew J. Dalby, Christopher C. West, Mhairi M. Harper, Enateri V. Alakpa, Laura Goldie |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Lineage (genetic) Cellular differentiation 02 engineering and technology Computational biology Biology 021001 nanoscience & nanotechnology 03 medical and health sciences Molecular level medicine.anatomical_structure Metabolomics medicine Pericyte Stem cell 0210 nano-technology 030304 developmental biology |
| Zdroj: | Pericytes ISBN: 9781071610558 |
| DOI: | 10.1007/978-1-0716-1056-5_5 |
| Popis: | We report the use of self-assembled peptide (F2/S) hydrogels and cellular metabolomics to identify a number of innate molecules that are integral to the metabolic processes which drive cellular differentiation of multipotent pericyte stem cells. The culture system relies solely on substrate mechanics to induce differentiation in the absence of traditional differentiation media and therefore is a non-invasive approach to assessing cellular behavior at the molecular level and identifying key metabolites in this process. This novel approach demonstrates that simple metabolites can provide an alternative means to direct stem cell differentiation and that biomaterials can be used to identify them simply and quickly. |
| Databáze: | OpenAIRE |
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