Effects of fibroblast growth factor 9 on steroidogenesis and control of FGFR2IIIc mRNA in porcine granulosa cells1,2
| Autor: | J.R. Evans, J. A. Williams, Nicole B. Schreiber, Leon J. Spicer |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Granulosa cell differentiation
endocrine system Chemistry Fibroblast growth factor receptor 2 Granulosa cell General Medicine Fibroblast growth factor receptor 4 Fibroblast growth factor receptor 3 Fibroblast growth factor Andrology FGF9 Genetics Animal Science and Zoology Granulosa cell proliferation Food Science |
| Zdroj: | Journal of Animal Science. 92:511-519 |
| ISSN: | 1525-3163 0021-8812 |
| DOI: | 10.2527/jas.2013-6989 |
| Popis: | The objectives of this study were to investigate the effects of fibroblast growth factor 9 (FGF9) on hormone-stimulated porcine granulosa cell proliferation and steroid production and to further elucidate the hormonal and developmental control of FGFR2IIIc gene expression in granulosa cells. Porcine ovaries were collected from a local slaughterhouse and granulosa cells were collected from small to medium (1 to 5 mm) follicles for 5 in vitro studies that were conducted. Cells were cultured for 48 h in 5% fetal calf serum plus 5% porcine serum and then treated with various combinations of FSH, IGF-I, FGF9, Sonic hedgehog (SHH), cortisol, PGE2, and/or wingless-type mouse mammary tumor virus integration site family member 5A (WNT5A) in serum-free medium for an additional 24 or 48 h. Medium was collected for analysis of steroid concentration via RIA, or RNA was collected for gene expression analysis of FGFR2IIIc via quantitative reverse transcription PCR. Fibroblast growth factor 9 stimulated (P 0.10) whereas IGF-I increased (P 0.10) on FGFR2IIIc gene expression whereas FGF9 in the presence of FSH and IGF-I inhibited (P < 0.05) FGFR2IIIc gene expression. In an in vivo study, granulosa cells from large (7 to 14 mm) follicles had greater (P < 0.05) abundance of FGFR2IIIc mRNA than small (1 to 3 mm) or medium (4 to 6 mm) follicles. In conclusion, IGF-I-induced FGFR2IIIc mRNA may be a mechanism for increased responses to FGF9 in FSH plus IGF-I-treated granulosa cells. Fibroblast growth factor 9 and IGF-I may work together as amplifiers of follicular growth and granulosa cell differentiation by stimulating estradiol production and concomitantly stimulating granulosa cell growth in pigs. |
| Databáze: | OpenAIRE |
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