Identification of whitefly transmitted tomato yellow leaf curl geminivirus from Iran and a survey of its distribution with molecular probes

Autor: A. Ahoonmanesh, M. R. Hajimorad, A. Kheyr-Pour, Masoud Bahar, M. A. Rezaian, V. Alavi, B. Gronenborn
Rok vydání: 1996
Předmět:
Zdroj: Plant Pathology. 45:418-425
ISSN: 1365-3059
0032-0862
Popis: MR. HAJIMORAD'*, A. KHEYR-POUR", V. ALAVJ", A. AHOONMANESH^,M.BAHAR'^t, M. A.REZAIAN'* and B. GRONENBORN''^ Plant Pests and Diseases Research Institute, PO Box 1454, Tehran-19395: and ^Department of PlantPathology, Esfahan University ofTeehnology. Esfahan; Iran"IfLstitut dcs Seiences Vegetales. CNRS. 91198 Gif-sur-Yvette Cedex, Franee^ of Horticulture, CSIRO. GPO Box 350, South Australia 5001. AustraliaA virtis causing a disease of tomato, prevaletit in the southern provinces of Iran, with symptoms of leaf-curling, stunting, reduction of leaf size, leaf corrugation, shortening of internodes and severe reductionin fruit yield, was shown to be transmissible to healthy tomato plants by grafting and by whiteflies{Beniisia (abaci), but not by sap inoculation. Geminivirus DNA was detected in extracts of diseasedtomato plants by dot-blot hybridization assays using as probes full-length cloned DNA of Australian,Italian {Sardinian) or Jordanian strains of tomato yellow leaf curl virus (TYLCV). Geminivirus coatprotein was detected in whitefly inoculated plants by dot immunobinding assay using polyclonalantibody raised against Jordanian TYLCV. A limited survey using the dot-blot hybridization assay forvirus detection indicated the presence of the virus in tomato-growing provinces of southern but notnorthern Iran. Whitefly transmission experiments to tomato under controlled greenhouse conditionsshowed that some isolates of TYLCV-like geminiviruses from different parts of Iran difFer insymptomatology.INTRODUCTIONA serious virus disease, which exhibits variousprominent symptoms such as upward curling ofleaf margins, stunting, reduction of leaf size atthe top of the plant, corrugated leaf, shorteningof internodes and severe reduction in fruit yield,has become prevalent in tomato fields all over theMiddle East (Makkouk & Laterrot, 1983).Similar symptoms have been described fromdiseased tomato plants in north and centralAfrica, south east Asia, Taiwan, Mexico, Italy(Sardinia), Spain, Australia, Dominican Repub-lic and Jamaica (Makkouk & Laterrot, 1983;Brown & Nelson, 1988; Czosnek et al., 1990;Kheyr-Pour et aL, 1991; Dry et al.. 1993;McGlashan et al., 1994; Noris et afA994\ Polston*Corresponding author.tPresent address: Department of Crop Protec-tion, Waitc Agricultural Research Institute, GlenOsmond, South Australia 5064, Australia.Accepted 15 November 1995.et al., 1994). The virus is transmitted by thewhitefly Bemisia tabaci (Cohen & Harpaz, 1964)but not mechanically (Cohen & Nitzany, 1966)and in all locations except Mexico has beencalled tomato yellow leaf curl geminivirus(TYLCV) (Brown & Nelson, 1988; Czosneket al., 1990; Kheyr-Pour et al., 1991; Dry et ai,1993; McGlashan et al.. 1994; Noris et al., 1994;Polston et ai., 1994). However, despite severalcommon characteristics between the virus foundin Mexico and TYLCV, the Mexican virus hasbeen called chino del tomate virus (Brown N Habibi, 1975; Javan-moghadam, 1993). In this report we describethe association of TYLCV with the disease inIran. We also indicate the distribution of the virusin Iran and describe symptom variation amongstvirus isolates from different parts of the country.
Databáze: OpenAIRE
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