63 EQUINE FOLLICLES MODULATE CORTISOL LEVELS AND CAPABILITY OF OOCYTES TO ADAPT TO STRESS SITUATIONS
| Autor: | S. O. Paiva, D. Scarlet, Melba O. Gastal, Eduardo L. Gastal, Natascha Ille, Benner Geraldo Alves, Christine Aurich, G. D. A. Gastal |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
biology Ovary Reproductive technology Oogenesis Follicular fluid Endocrinology medicine.anatomical_structure Reproductive Medicine Transcortin Internal medicine Follicular phase Genetics biology.protein medicine Animal Science and Zoology Folliculogenesis Molecular Biology Developmental Biology Biotechnology Hydrocortisone medicine.drug |
| Zdroj: | Reproduction, Fertility and Development. 28:161 |
| ISSN: | 1031-3613 |
| DOI: | 10.1071/rdv28n2ab63 |
| Popis: | Glucocorticoids are mediators of the systemic stress response. Acute or chronic stress characterised by high cortisol concentrations in the periphery impairs reproductive function in a variety of species and therefore may affect fertility. The ovary has been shown to be a target tissue for glucocorticoids in many species, including the mare. This study hypothesised that the equine ovary possesses internal mechanisms to modulate cortisol activity and that supraphysiologic levels of glucocorticoids do not affect oocyte IVM rates. Light horse mares (n = 9) were used in this study. Growing follicles from an induced follicular wave were divided into the following groups: G1: 5–9 mm, G2: 10–14 mm, G3: 15–19 mm, G4: 20–24 mm, and G5: ≥25 mm. Follicular fluid (FF) and compact cumulus‐oocyte complexes (COCs) were obtained by ultrasound-guided transvaginal aspiration. Blood samples were collected at the beginning and the end of every aspiration session. Cortisol (DE1887, Demeditec, Kiel-Wellsee, Germany), progesterone (ADI-901–011, Enzo Life Sciences, Farmingdale, NY, USA), and corticosteroid binding globulin (CBG, MBS047353, MyBioSource, San Diego, CA, USA) concentrations were determined by ELISA. COCs (n = 80) were randomly distributed to either the control group (DMEM-F12+ medium) or the following hydrocortisone treatment groups: 0.1 µg mL–1, 1 µg mL–1, 5 µg mL–1, 10 µg mL–1. Maturation rate was assessed 30 h after incubation. Statistical analysis was performed with the SPSS Statistics 22 software. Data were analysed using one-way ANOVA, Pearson correlation, and chi-squared test. Cortisol (115.4 ± 13.3 ng mL–1) and progesterone (22.1 ± 3.1 ng mL–1) FF concentrations were higher (P |
| Databáze: | OpenAIRE |
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