PERMANENT GENETIC RESOURCES: Eighteen new polymorphic microsatellite markers for the endangered Florida manatee, Trichechus manatus latirostris

Autor:	Marta A. Rodriguez-lopez, John E. Reynolds, Susan L. Carney, Elsa M. Haubold, Seifu Seyoum, Michael D. Tringali, Michelle C. Davis
Rok vydání:	2008
Předmět:	Ecology Endangered species Zoology Trichechus manatus latirostris Locus (genetics) Biology Genetic resources Genotype Genetics Microsatellite Polymorphic Microsatellite Marker Florida manatee Ecology Evolution Behavior and Systematics Biotechnology
Zdroj:	Molecular Ecology Resources. 8:328-331
ISSN:	1755-098X
DOI:	10.1111/j.1471-8286.2007.01944.x
Popis:	Here we describe 18 polymorphic microsatellite loci for Trichechus manatus latirostris (Florida manatee), isolated using a polymerase chain reaction-based technique. The number of alleles at each locus ranged from two to four (mean = 2.5) in specimens from southwest (n = 58) and northeast (n = 58) Florida. Expected and observed heterozygosities ranged from 0.11 to 0.67 (mean = 0.35) and from 0.02 to 0.78 (mean = 0.34), respectively. Departures from Hardy-Weinberg equilibrium occurred at two loci. There was no evidence of genotypic disequilibrium for any pair of loci. For individual identification, mean random-mating and θ-corrected match probabilities were 9.36 × 10(-7) and 1.95 × 10(-6) , respectively.
Databáze:	OpenAIRE
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