Phytase-Fe3O4 nanoparticles-loaded microcosms of silica for catalytic remediation of phytate-phosphorous from eutrophic water bodies
| Autor: | H. A. Modi, Gayatri Dave |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Phytic acid
Chemistry Environmental remediation Health Toxicology and Mutagenesis chemistry.chemical_element General Medicine 010501 environmental sciences Phosphate 01 natural sciences Pollution Nitrogen Catalysis chemistry.chemical_compound Adsorption Environmental Chemistry Phytase Microcosm 0105 earth and related environmental sciences Nuclear chemistry |
| Zdroj: | Environmental Science and Pollution Research. 26:14988-15000 |
| ISSN: | 1614-7499 0944-1344 |
| Popis: | Agriculture P management practices elevate the level of inorganic phosphates in soil that results in phosphorous (P) seepage into water-bodies. This is one of the key factors that have accelerated the menace of eutrophication. Phytic acid (phytate)-P-rich plant metabolite is infamous for its anti-nutrient activity and regularly oozing in to environment though discharge of mono-gastric animals. That has amplified the magnitudes of eutrophication. In this work, for catalysis of phytate-P, the metal-organic framework fabricated towards metal oxides (Fe3O4) and phytase in highly ordered microcosms of silica was employed. The synthesized framework was characterized through transmission electron microscopy (TEM) and nitrogen isotherm analysis. Average pore diameter of synthesized bisect oval shaped structures was measured around ≈200 nm. Herein, phytase and Fe3O4 nanoparticles were loaded to the cavities of microcosms through glutaraldehyde-mediated crosslinking. Whereas Fe3O4 nanoparticles act as nano-absorbents that adsorb P liberated from phytase-mediated catalysis of phytate. Kinetic analysis of free and loaded phytase has shown relatively small reduction in catalytic efficiency. These loaded microcosms have removed 60–80% of phytate-phosphate. The optimized process has reduced the growth of photoautotrophs by 50%. Additionally the magnet-assisted separation of loaded microcosms eased the reapplication of loaded microcosms tested for six independent instances. The primary studies conducted to evaluate the geno-toxicity of loaded microcosms have not shown any harmful effect on the process like cell division and seed germination. The efficacy of this method has evaluated towards on-field testing in Changa (Gujarat, India) lake. |
| Databáze: | OpenAIRE |
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