Purification of (S)-3-cyano-5-methylhexanoic acid from bioconversion broth using an acetone/ammonium sulfate aqueous two-phase system

Autor:	Zhe-Ming Wu, Chang-Ling Hao, Tao Tong, Ren-Chao Zheng, Yu-Guo Zheng
Rok vydání:	2020
Předmět:	chemistry.chemical_classification Ammonium sulfate Aqueous solution Bioconversion Extraction (chemistry) Aqueous two-phase system Salt (chemistry) Bioengineering Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound chemistry Acetone Methanol Nuclear chemistry
Zdroj:	Process Biochemistry. 89:186-192
ISSN:	1359-5113
DOI:	10.1016/j.procbio.2019.10.023
Popis:	(S)-3-Cyano-5-methylhexanoic acid ((S)-CMHA) is the key chiral intermediate of pregabalin. In this paper, an aqueous two-phase system (ATPS) was developed to extract (S)-CMHA from nitrilase-catalyzed bioconversion broth. Inorganic salts and hydrophilic solvents were screened to form ATPS, among which an acetone/ammonium sulfate ATPS was investigated in detail, including phase diagram, effect of phase composition and stability of (S)-CMHA. The maximum product recovery of 99.15% was obtained by an optimized ATPS system composed of 15% (w/w) ammonium sulfate and 35% (w/w) acetone with the removal of 99% cells and 86.27% proteins. The total (S)-CMHA yield reached 92.11% after back-extraction. The recycling use of ammonium sulfate was investigated, and 93.10% of salt in the salt-rich phase was recovered with the addition of methanol. The results demonstrated the efficiency of the two-step extraction process for separation of (S)-CMHA.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0d8cc069a272ddec6df109bc28127a76 https://doi.org/10.1016/j.procbio.2019.10.023 Zobrazit plný text záznamu Full Text from ScienceDirect