Engraftment after infusion of CD34 enriched harrow cells

Autor: Irwin D. Bernstein, C. Dean Buckner, Juan Garcia-Lopez, John A. Hansen, William I. Bensinger, Gary Spitzer, Berenson Rj, Robert G. Andrews
Rok vydání: 1992
Předmět:
Zdroj: The International Journal of Cell Cloning. 10:176-178
ISSN: 1549-4918
0737-1454
DOI: 10.1002/stem.5530100759
Popis: The CD34 antigen, expressed on 1–4% of marrow cells obtained from humans and primates, includes virtually all committed and primitive progenitors detected by in vitro assays. Previous studies in baboons have demonstrated that the infusion of small numbers of CD34 enriched marrow cells leads to full hematopoetic reconstitution after lethal irradiation. Because the CD34 antigen was not detected on breast cancer or neuroblastoma cells it was possible to use antibodies to the CD34 antigen to separate CD34 enriched cells from the marrows of such patients. Thirteen patients (11 with metastatic breast cancer and 2 with neuroblastoma) had 2.5–22.0 × 109 nucleated marrow cells processed for CD34 enrichment. We recovered 50–260 × 106 nucleated cells that adhered to the column which were 35–92% CD34 positive. Patients received 1.0–5.2 × 106 CD34 enriched cells/kg following myeloablative therapy. Ten of 13 patients, who survived long enough to be evaluable for engraftment, achieved 500 neutrophils/mm3 between days 15–45 and a self-supporting platelet count of 20,000/mm3 between days 23–66. We conclude that small numbers of CD34 enriched marrow cells are capable of autologous hematopoetic reconstitution in humans.
Databáze: OpenAIRE
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