Transient gus expression in zygotic embryos ofTaxus Brevifolia

Autor:	Ellen C. Luan, Mark G. Bolyard, Susan E. Allen
Rok vydání:	1996
Předmět:	Expression vector fungi Embryogenesis food and beverages Embryo Plant Science Biology biology.organism_classification Molecular biology Taxus brevifolia Tissue culture Gene expression Botany Gene Developmental biology Biotechnology
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 32:81-85
ISSN:	1475-2689 1054-5476
Popis:	Germinated zygotic embryos of Pacific yew (Taxus brevifolia) were transiently transformed with the expression vector pBl121, as demonstrated by expression of the β-glucuronidase (GUS) gene. Embryos exhibited sectors of blue color following treatment with DNA and dimethyl sulfoxide (optimal concentration of 2.25%). However, transient expression depended on the presence of ions in the buffer, as no GUS activity was observed when deionized water was used in place of other buffers. GUS gene expression was dependent on the developmental stage of the embryo, since the frequency of GUS expression was elevated in embryos incubated with the expression vector during a period from 4 to 8 wk after culture of DCR medium. In addition, the utility of using zygotic embryos from mature seeds for tissue culture and transient expression experiments was also examined.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::0d6ec18455a0e5da7c71474f84b444fa https://doi.org/10.1007/bf02823135 Zobrazit plný text záznamu Full text from SpringerLink