| Autor: |
J. Heckenkamp, Michael Gawenda, Jan Brunkwall, S. Kossodo, G. M. LaMuraglia |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Deutsche Gesellschaft für Chirurgie ISBN: 9783540417187 |
| Popis: |
Background: Ionizing irradiation is a successful approach to inhibit vascular restenosis. The specific mechanisms by which it modulates the post-interventional vascular injury response have not been fully elucidated. This study investigates how γ-irradiation (γ-RT) affects vascular cell invasive migration, a key factor in the development of restenosis. Methods: Smooth muscle cell (SMC) migration (calibrated microscopy) into a three-dimensional collagen matrix and the release of metalloproteinases (ELISA, zymography) were quantitated after γ-RT (20 Gy). The γ-RT effects on fibronectin bound TGF-β were elucidated by quantitating the proliferative activity of untreated SMCs, seeded on the irradiated TGF-β ([3H]-thymidine incorporation). Molecular weight changes in the collagen matrix after γ-RT were also assessed (SDS-PAGE). Results: γ-RT of SMCs decreased invasive migration by 63% at 7 days (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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