Fabrication of surfactant-free quercetin-loaded PLGA nanoparticles: evaluation of hepatoprotective efficacy by nuclear scintigraphy
| Autor: | Raghuvir H Gaonkar, Dipankar Chattopadhyay, Satbir S. Sachdeva, Soumya Ganguly, Shantanu Ganguly, Samarendu Sinha, Sankha Chattopadhyay, Mita Chatterjee Debnath, Amit Gupta |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
Nanoparticle Bioengineering CCL4 02 engineering and technology General Chemistry Pharmacology 021001 nanoscience & nanotechnology Condensed Matter Physics Atomic and Molecular Physics and Optics In vitro Excretion 03 medical and health sciences PLGA chemistry.chemical_compound 0302 clinical medicine chemistry 030220 oncology & carcinogenesis Modeling and Simulation Nuclear scintigraphy Distribution (pharmacology) General Materials Science 0210 nano-technology Quercetin |
| Zdroj: | Journal of Nanoparticle Research. 18 |
| ISSN: | 1572-896X 1388-0764 |
| DOI: | 10.1007/s11051-016-3504-0 |
| Popis: | The purpose of this study was to develop surfactant-free quercetin-loaded PLGA nanoparticles (Qr-NPs) and investigate the hepatoprotective efficacy of the product non-invasively by nuclear scintigraphy. The nanoparticles were prepared using PLGA by dialysis method and ranged in size between 50 and 250 nm with a narrow range of distribution. They were found to arrive at the fenestra of liver sinusoidal epithelium for accumulation. The sizes of nanoparticles (batch S1) were optimal to reach the target and offer enough protection of the hepatocytes degenerated by CCl4 intoxication as determined by various biochemical and histopathological tests. In vitro studies exhibited the cytotoxic effect of the formulation against HepG2 cell line. The hepatoprotective efficacy of Qr-NPs evaluated non-invasively by nuclear scintigraphic technique using 99mTc-labelled sulphur colloid revealed abnormality in liver at the area of decreased uptake in rats of CCl4-treated group, which disappeared in Qr-NP-treated group. In dynamic studies with 99mTc-mebrofenin, excretion was severely impaired in CCl4-treated group but was moderate in drug-treated group, proving the recovery of animals from damage. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink