Menthol as anesthetic for juvenile Lophiosilurus alexandri: Induction and recovery time, ventilatory frequency, hematology and blood biochemistry

Autor: Imaculada de Morais Carvalho Ananias, Andre Lima Ferreira, Edenilce de Fátima Ferreira Martins, Ronald Kennedy Luz, Deliane Cristina Costa, Caroline Lopes de Melo, Rodrigo Takata
Rok vydání: 2022
Předmět:
Zdroj: Aquaculture. 546:737373
ISSN: 0044-8486
DOI: 10.1016/j.aquaculture.2021.737373
Popis: Anesthesia is an indispensable procedure during the handling of fish. This work evaluated the effects of menthol as anesthetic for juvenile Lophiosilurus alexandri. Menthol concentrations of 0 (control), 25, 50, 150, 300 and 450 mg L−1 were tested using 14 animals for each concentration, with each fish being a replicate. Blood was sampled from seven of the juveniles in each group shortly after anesthetic induction and from the other seven at 24 h post-anesthesia and procedures to collect biometric measures, i.e., handling and air exposure. Induction time showed a linear response plateau effect (LRP) with an inflection point of 142 mg L−1, while a dose-response relationship was observed among concentrations for recovery time. Ventilatory frequency (VF) during anesthetic induction had a LRP effect with an inflection point of 127 mg L−1, while during recovery it had a quadratic effect with a maximum point of 305.45 mg L−1. Fish anesthetized with 50 mg L−1 of menthol had the lowest hematocrit values (P
Databáze: OpenAIRE