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Three in vitro experiments were conducted to determine the rumen fermentability of Mucuna (M) pruriens (24 g 3,4-dihydroxy- l -phenylalanine ( l -dopa)/kg dry matter (DM) and soybean meal treated with (SBD) or without (SB) 138 g l -dopa/kg DM). Additional objectives were to determine if l -dopa inhibits rumen fermentation, and if ruminal microbes can adapt to l -dopa or M. In Experiment 1, ground (1 mm) substrates were incubated in triplicate at 38 °C in 9 ml nutrient media and 1 ml rumen fluid in a series of six, 48 h, consecutive batch cultures. The first culture was inoculated with rumen fluid from two donor cows. Subsequent cultures were inoculated with fluid (1 ml) from the previous culture. The DM digestibility (DMD, 616 g/kg vs . 540 g/kg; P vs . 44.2 ml/g DM; P versus SB but similar for SB and SBD (540 g/kg vs . 554 g/kg and 44.2 ml/g DM vs . 43.5 ml/g DM, respectively). The slopes of the relationships between DMD (g/kg) or gas production (ml/g DM) and fermentation period were not reduced by fermenting M (−0.014 DMD slope; 2.28 gas production slope) or SBD (−0.014 DMD slope; 0.459 gas production slope), instead of SB (−0.002 DMD slope; 1.039 gas production slope), indicating microbial adaptation to M and SBD. Total volatile fatty acid concentration (VFA; 53.7, 54.9 and 54.9 mmol/l) and molar proportions of VFA were similar among substrates. Gas production kinetics of M versus SB (Experiment 2), and SB versus SBD (Experiment 3) were also measured after substrates were incubated in triplicate in buffered rumen fluid for 24 h using a non-linear exponential model to fit the data. Residual l -dopa was measured after separate fermentation of substrates in triplicate for 0, 4, 8, 16 and 24 h. Fermentation of M versus SB produced more ( P vs . 100 ml/g DM) and total VFA (203 mmol/l vs . 180 mmol/l) and a lower ( P vs . 1.87; P l -dopa to SB increased ( P vs . 200 ml/g DM), and total VFA concentration (132 mmol/l vs . 188 mmol/l), but reduced ( P vs . 0.05 ml/h). The concentration of l -dopa in fermented M and SBD decreased by 53 and 47%, respectively during fermentation. In conclusion, M was more fermented than SB and degradation of l -dopa during ruminal fermentation and microbial adaptation to l -dopa were confirmed. Adding l -dopa to SB did not impair ruminal fermentation. |
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