e0155 Smoking treatment increase serum AGEs Level and have effects on expression of ICAM-1 in vascular endothelial cells of rat
| Autor: | Naifen Liu, Yungen Jiao |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Pathology ICAM-1 Tobacco use business.industry medicine.medical_treatment Reverse transcription polymerase chain reaction chemistry.chemical_compound Endocrinology chemistry Puerarin Internal medicine medicine Smoking cessation Immunohistochemistry Advanced glycation end-product Smoking treatment Cardiology and Cardiovascular Medicine business |
| Zdroj: | Heart. 96:A50-A50 |
| ISSN: | 1355-6037 |
| DOI: | 10.1136/hrt.2010.208967.155 |
| Popis: | Objective To investigate whether smoking can increase serum advanced glycosylation end products (AGEs) level and have effects on expression of intercellular cell adhesion molecule-1 (ICAM-1) in vascular endothelial cells of rat. Methods Male SD rats (n=138) were randomly assigned to five groups according to duration of smoking treatment: 2-week group, 4-week group, 6-week group, 8-week group, smoking cessation group. The rats of following groups, that is 2w, 4w, 6w and 8w groups were further randomly divided into five subgroups according to intervening condition: control subgroup, smoking treatment for 1 h/per day subgroup, smoking treatment for 0.5 h/per day subgroup, aminoguanidine hydrochloride subgroup, puerarin subgroup; the rats of smoking cessation group were further randomly divided into three subgroups according to duration of smoking cessation: smoking cessation 2 weeks subgroup, smoking cessation 4 weeks subgroup and smoking cessation 6 weeks subgroup. The rats of 2w, 4w, 6w and 8w groups were sacrificed after smoking treatment for 2, 4, 6 and 8 weeks respectively; the rats of smoking cessation group were sacrificed after smoking treatment for 8 weeks and then cease-smoking for 2, 4, and 6 weeks respectively. Serum AGEs levels of each rat were assayed by fluorescent method. ICAM-1 mRNA and protein of vascular endothelial cells were determined by semiquantitative RT-PCR (Reverse transcription PCR) and immunohistochemistry. Results Serum AGEs levels of all SM1 subgroups rats were increased after smoking treatment for 2 weeks (p Conclusions Smoking treatment increase serum AGEs level in rat. Cigarette-induced AGEs play roles in the augmented expression of ICAM-1 in vascular endothelial cells of rat with smoking treatment. Aminoguanidine hydrochloride, puerarin and smoking cessation contribute to the decrease of serum AGEs level and the expression of ICAM-1 in vascular endothelial cells of rat. |
| Databáze: | OpenAIRE |
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