Autor: Jérôme Liaigre, Danièle Bouhours, Jeanne Naulet, Jean-François Bouhours, Daniel Maume
Rok vydání: 1997
Předmět:
Zdroj: Glycoconjugate Journal. 14:29-38
ISSN: 0282-0080
Popis: Immunodetection of thin layer chromatograms of neutral glycosphingolipids of pig kidney cortex with a polyclonal antibody directed against the Galα1-3Gal determinant revealed several glycosphingolipids reacting with different intensities. A minor glycosphingolipid was isolated by preparative high performance thin layer chromatography. It was characterized as a type 2 hexaglycosylceramide with the following structure Galα1-3Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4Glcβ1-Cer by fast atom bombardment- and desorption-chemical ionization-mass spectrometry, methylation analysis and hydrolysis with α-galactosidase followed by immunostaining with an anti-Lewisx monoclonal antibody. The proton NMR spectrum was found compatible with the proposed structure. Two other glycosphingolipids carrying the new determinant were partially characterized as an octa- and a branched-dodecaglycosylceramide. The expression of the Galα1-3Lewisx determinant appeared to be developmentally regulated as it increased with age. The characterization of Galα1-3Lex in pig kidney indicates a new epitope capable of recognition by human natural antibodies in the context of xenotransplantation of pig organs to man. It also adds new members to the family of Lex-based glycolipids. Abbreviations: HPTLC, high performance thin layer chromatography; FAB-MS, fast atom bombardment mass spectrometry; DCI, desorption-chemical ionization; Me2SO-d6, hexadeuterated dimethyl sulfoxide
Databáze: OpenAIRE
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