Alterations to the catalytic properties of polyphenoloxidase in detergentless microemulsions and ternary water-organic solvent mixtures
| Autor: | Iqbal Gill, Evgeny N. Vulfson, Barry A. Law, Igor A. Kozlov, Peter W. Goodenough, Ghulam Ahmed |
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| Rok vydání: | 1991 |
| Předmět: |
Ternary numeral system
Immobilized enzyme Substrate (chemistry) Bioengineering General Medicine Applied Microbiology and Biotechnology Toluene Catalysis body regions Hexane chemistry.chemical_compound chemistry parasitic diseases Organic chemistry Microemulsion Ternary operation Biotechnology Nuclear chemistry |
| Zdroj: | Biotechnology Letters. 13:91-96 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/bf01030457 |
| Popis: | The catalytic properties of mushroom polyphenoloxidase could be substantially altered by entrapment into hexane- and toluene-based microemulsions stabilized with isopropanol. The fast irreversible inactivation and drastic substrate inhibition of the enzyme were significantly reduced in detergentless microemulsions in comparison to conventional aqueous media. Similar changes in the catalytic behavior of polyphenoloxidase were observed in the normal ternary solutions of hexane-(toluene)-isopropanol-water, and in the H-bonded aggregates of isopropanol and water in toluene, but not in hexane. |
| Databáze: | OpenAIRE |
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